ABSTRACT
<p><b>OBJECTIVE</b>To provide experimental evidence for development of human cytomegalovirus (HCMV) nucleic acid vaccine, HCMV surface protein (gB), membrane protein (pp150), and gB-pp150 fused gene eukaryotic expression vector were constructed.</p><p><b>METHODS</b>gB and pp150 genes were amplified and fused into gB-pp150, then were cloned into pcDNA 3.1 (+) to obtain recombinant expression plasmids pcDNA 3.1 (+) -gB, pcDNA 3.1 (+) -pp150 and pcDNA 3.1 (+) -gB-pp150, which were encapsulated with chitosan. Mouse were vaccinated and the humoral and cell immune response were determined by ELISA, specific proliferative response of plenic lymphocytes.</p><p><b>RESULTS</b>The gB, pp150 and gB-pp150 fusion gene eukaryotic expression vector were successfully constructed. The antibodies A value induced by pcDNA3.1(+) -gB or pcDNA3.1 (+) -gB-pp150 were much higher than that of pcDNA3.1 (+) (P < 0.01). The IFN-gamma levels induced by pcDNA3.1 (+) -pp150 and pcDNA3.1 (+) -gB-pp150 were significantly higher than that of pcDNA3.1 (+). There are significant diference between the stimulating indexes of pcDNA3.1(+) -pp150 or pcDNA3.1 (+) -gB-pp150 immunized and normal mice.</p><p><b>CONCLUSION</b>The DNA vaccine pcDNA3.1 (+) -gB can induce significant humoral immunity response, and pcDNA3.1 (+) -pp150 can induce high cellular immune response, whereas pcDNA3.1 (+) -gB-pp150 can induce both humoral and cellar immune responses in BALB/c mice.</p>
Subject(s)
Animals , Humans , Mice , Cytomegalovirus , Genetics , Allergy and Immunology , Immunity, Cellular , Allergy and Immunology , Immunization , Mice, Inbred BALB C , Recombinant Fusion Proteins , Allergy and Immunology , Vaccination , Vaccines, DNA , Allergy and Immunology , Viral Envelope Proteins , Allergy and ImmunologyABSTRACT
Polysaccharides are important large molecules in the body. It has many functions such as activating the lymphocytes like T, B, NK and Mφ cells, promoting the secretion of cytokines and antibodies through binding to the CR3 receptor of lymphocytes, affecting the concentrations of cellar [Ca2+]i, cAMP, NO and PGE2, and promoting the gene expression of cytokines. Its function also relate to its molecule weight and structure.